站内搜索

重庆医科大学病毒性肝炎研究所

Institute for Viral Hepatitis,Chongqing Medical University


暨感染性疾病分子生物学教育部重点实验室

Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education


任红教授团队发表TGFβ1- miR-140-5p axis mediated up-regulation of Flap Endonuclease 1 promotes epithelial-mesenchymal transition in hepatocellular carcinoma
发布时间:2019-12-13 08:47:51

TGFβ1- miR-140-5p axis mediated up-regulation of Flap Endonuclease 1 promotes epithelial-mesenchymal transition in hepatocellular carcinoma

Chuanfei Li  ,  Di Zhou  ,  Hao Hong  ,  Shuangyan Yang  ,  Li Zhang  ,  Shiying Li  ,  Peng Hu , Hong Ren ,  Zhechuan Mei  ,  Hui Tang


received: May 7, 2019 ; accepted: July 30, 2019 ; published: August 10, 2019


PMID31402791 [ - in process]

PMCID

PMC6710057

Abstract

Flap Endonuclease 1 (FEN1) is a known oncogene in an array of cancers, but its role in hepatocellular carcinoma (HCC) remains obscure. In this study, we report that FEN1 expression was elevated in the Cancer Genome Atlas (TCGA) database which was verified in HCC tissue and hepatoma cell lines. Pearson correlation analysis indicated that FEN1 was involved in HCC metastasis. We demonstrated that FEN1 silencing inhibits HCC cell epithelial-mesenchymal transition (EMT), invasion and migration in vitro and significantly suppressed tumor growth and metastasis in vivo. Conversely, FEN1 overexpression in HCC cells enhanced these metastatic processes. We further confirmed that FEN1 was a direct target of miR-140-5p, which was down-regulated in HCC tissues, and negatively correlated with FEN1 expression. Moreover, low miR-140-5p levels and high FEN1 expression predicted a poor clinical outcome. The effects of FEN1 overexpression could be partially abolished by miR-140-5p. miR-140-5p down-regulation and FEN1 overexpression were observed in a TGFβ1 induced EMT model. TGFβ1 mediated EMT could be blocked by miR-140-5p overexpression or FEN1 silencing. Taken together, our findings suggest that FEN1 is regulated by the TGFβ1- miR-140-5p axis and promotes EMT in HCC.

重庆医科大学病毒性肝炎研究所 版权所有
地址:重庆临江路74号 邮编:400010 电话:023-63893396